New calibration curves for biological dosimetry in Cuba
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Abstract
In this paper we show the dose response curves in vitro obtained at first time in Cuba, with several biological indicators for radiation damage: rings in premature chromosome condensation (PCC-R); PCC index and -H2AX foci, all of these in peripheral human lymphocytes. These curves cover an interval of doses between 0.1 Gy to 25 Gy, several radiation qualities and times post-exposition, strengthening the capabilities of the Cuban laboratory to give answer to the radiological emergencies and in the same way increase its contribution to elucidate any radiological abnormal event take place at the region where the main indicator at the present time is the dicentric assay in lymphocytes of peripheral blood.
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How to Cite
Lamadrid Boada, A. I., Mandina Cardoso, T., González Mesa, J. E., & Romero Aguilera, I. (1). New calibration curves for biological dosimetry in Cuba. Nucleus, (49). Retrieved from http://nucleus.cubaenergia.cu/index.php/nucleus/article/view/543
Section
Ciencias Nucleares
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References
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4. LAMADRID AI, GARCIA O, DELBOS M, et. al. PCC-ring induction in human lymphocytes exposed to gamma and neutron irradiation. J. Radiat. Res. 2007; 48(1): 1-6.
5. GOTOH E, TANNO Y, TAKAKURA K. Simple biodosimetry method for use in cases of high dose radiation exposure that scores the chromosome number of giemsa-stained drug-induced prematurely condensed chromosomes (PCC). Int J Radiat Biol. 2005; 81(1): 33-40.
6. LAMADRID AI, GONZALEZ JE, GARCIA O, et. al. Prematurely condensed chromosome rings after neutron irradiation of human lymphocytes. J. Radiat. Res. 2011. In press.
7. LÖBRICH M, RIEF N, KÜHNE M, et al. In vivo formation and repair of DNA double-strand breaks after computed tomography examinations. Proc. Natl. Acad. Sci USA. 2005; 102(25): 8984-8989.
8. ROTHKAMM K, BALROOP SH, SHEKHDAR J, et al. Leukocyte DNA damage after multi-detector row CT: a quantitative biomarker of low-level radiation exposure. Radiology. 2007; 242(1): 244-251.
9. ROCH-LEFÈVRE SH, MANDINA T, VOISIN PA, et. al. Quantification of -H2AX foci in human lymphocytes: a method for biological dosimetry after ionizing radiation exposure. Rad. Res. 2010; 174(2): 185-194.
10. REDON CHE, DICKEY JS, BONNER W M, SEDELNIKOVA OA. -H2AX as a biomarker of DNA damage induced by ionizing radiation in human peripheral blood lymphocytes and artifi cial skin. Advances in Space Research. 2009; 43(8): 1171-1178.
11. PAPWORTH DG. Appendix in: Savage J.R.K. Sites of radiation induced chromosome exchanges. Curr. Top. Radiat. Res. 1970; 6: 129-194.
12. PAPWORTH DG. Curve fitting by maximum likelihood. Radiat. Bot. 1975; 15: 127-131.
13. VAURIJOUX A, GRUEL G, POUZOULET F, et. al. Strategy for population triage based on dicentric analysis. Radiat. Res. 2009; 171(5): 541-548.
14. LINDHOLM C, et. al. Premature chromosome condensation (PCC) assay for dose assesment in mass casualty accidents. Radiat. Res. 2010; 173 (1): 71-78.
15. HAYATA I, KANDA R, MINAMIHISAMATSU M, et. al. Cytogenetical dose estimation for 3 severely exposed patients in the JCO critically accident in Tokaimura. J. Radiat. Res. 2001; 42(Suppl): S-149-S-155.
16. BEELS L, WERBROUCK J, HUBERT TH. Dose response and repair kinetics of c-H2AX foci induced by in vitro irradiation of whole blood and T-lymphocytes with X- and -radiation. Int. J. Radiat. Biol. 2010; 86(9): 760–768.
2. NORMAN A, SASAKI MS. Chromosome-exchange aberrations in human lymphocytes. Int. J. Radiat. Biol. 1967; 11(4): 321-328.
3. KANDA R, HAYATA J, LLOYD DC. Easy biodosimetry for high-dose radiation exposures using drug-induced, prematurely condensed chromosomes. Int. J. Radiat. Biol. 1999; 75(4): 441-446.
4. LAMADRID AI, GARCIA O, DELBOS M, et. al. PCC-ring induction in human lymphocytes exposed to gamma and neutron irradiation. J. Radiat. Res. 2007; 48(1): 1-6.
5. GOTOH E, TANNO Y, TAKAKURA K. Simple biodosimetry method for use in cases of high dose radiation exposure that scores the chromosome number of giemsa-stained drug-induced prematurely condensed chromosomes (PCC). Int J Radiat Biol. 2005; 81(1): 33-40.
6. LAMADRID AI, GONZALEZ JE, GARCIA O, et. al. Prematurely condensed chromosome rings after neutron irradiation of human lymphocytes. J. Radiat. Res. 2011. In press.
7. LÖBRICH M, RIEF N, KÜHNE M, et al. In vivo formation and repair of DNA double-strand breaks after computed tomography examinations. Proc. Natl. Acad. Sci USA. 2005; 102(25): 8984-8989.
8. ROTHKAMM K, BALROOP SH, SHEKHDAR J, et al. Leukocyte DNA damage after multi-detector row CT: a quantitative biomarker of low-level radiation exposure. Radiology. 2007; 242(1): 244-251.
9. ROCH-LEFÈVRE SH, MANDINA T, VOISIN PA, et. al. Quantification of -H2AX foci in human lymphocytes: a method for biological dosimetry after ionizing radiation exposure. Rad. Res. 2010; 174(2): 185-194.
10. REDON CHE, DICKEY JS, BONNER W M, SEDELNIKOVA OA. -H2AX as a biomarker of DNA damage induced by ionizing radiation in human peripheral blood lymphocytes and artifi cial skin. Advances in Space Research. 2009; 43(8): 1171-1178.
11. PAPWORTH DG. Appendix in: Savage J.R.K. Sites of radiation induced chromosome exchanges. Curr. Top. Radiat. Res. 1970; 6: 129-194.
12. PAPWORTH DG. Curve fitting by maximum likelihood. Radiat. Bot. 1975; 15: 127-131.
13. VAURIJOUX A, GRUEL G, POUZOULET F, et. al. Strategy for population triage based on dicentric analysis. Radiat. Res. 2009; 171(5): 541-548.
14. LINDHOLM C, et. al. Premature chromosome condensation (PCC) assay for dose assesment in mass casualty accidents. Radiat. Res. 2010; 173 (1): 71-78.
15. HAYATA I, KANDA R, MINAMIHISAMATSU M, et. al. Cytogenetical dose estimation for 3 severely exposed patients in the JCO critically accident in Tokaimura. J. Radiat. Res. 2001; 42(Suppl): S-149-S-155.
16. BEELS L, WERBROUCK J, HUBERT TH. Dose response and repair kinetics of c-H2AX foci induced by in vitro irradiation of whole blood and T-lymphocytes with X- and -radiation. Int. J. Radiat. Biol. 2010; 86(9): 760–768.