Preparation of -macroaggregated albumin for lung perfusion studies

Main Article Content

Yumisley Alfonso Marín
Alejandro Rivero Santamaría
Ignacio Hernández González

Abstract

The present paper shows the design, optimization, quality control for testing radiochemical purity, biological quality and realtime stability studies of a preparation of macroaggregated albumin from the controlled denaturation of HSA. An experimental design with a factorial plan was carried out in order to determine the ideal conditions for the preparation. The results showed that variations of albumin concentrations within the range of 515
mg/mL were irrelevant, however, the final pH of the mixture and the stirring speed were significant variables, as well as their interaction. Formulations with particles of diameter between 11 and 80 µ were obtained with the conditions selected in the experimental design which met the internationally established criteria for
radiochemical purity, sterility and apyrogenicity. The biodistribution in experimental animals showed good quality lung images. The produced lots maintained physical, radiochemical, biological and microbiological stability during 90 days of storage at 4°C.

Article Details

How to Cite
Alfonso Marín, Y., Rivero Santamaría, A., & Hernández González, I. (1). Preparation of -macroaggregated albumin for lung perfusion studies. Nucleus, (45). Retrieved from http://nucleus.cubaenergia.cu/index.php/nucleus/article/view/519
Section
Ciencias Nucleares

References

[1] RAMZI D, KENNETH V, LEEPER M. DVT and Pulmonary Embolism: Part II. Treatment and Prevention. Am Fam. Physician. 2004; 69: 2841-8.
[2] TAPSON VF. Acute Pulmonary Embolism. N Engl J Med. 2008; 358(10): 1037-1052.
[3] CONTHE P, LOBOS JM, ALONSO A. Tromboembolismo Pulmonar. Formación Médica Continuada. 1998; 5(2): 89-97.
[4] HEIT JA, COHEN AT, ANDERSON FA. VTE Impact Assessment Group. Estimated annual number of incident and recurrent, non-fatal and fatal venous thromboembolism (VTE) events in the US. ASH Annual Meeting Abstracts. 2005. p. 267.
[5] SILVERSTEIN MD, HEIT JA, MOHR DN, et. al. Trends in the incidence of deep vein thrombosis and pulmonary embolism: a 25-year population-based study. Arch Intern. Med. 1998; 158(6): 585-593.
[6] PÉREZ RODRÍGUEZ EA. ¿Ha sustituido la tomografía computarizada helicoidal a la gammagrafía de perfusión en el diagnóstico del Tromboembolismo pulmonar?. Rev. Pat. Resp. 2000; (3): 21-22.
[7] República de Cuba. Anuario estadístico de salud 2007. MINSAP: Dirección nacional de estadística, 2007. p. 25.
[8] SCHOEPF UJ, CASTELLO P. CT angiography for diagnosis of pulmonary embolism: state of art. Radiol. 2004; 230(2): 329-337.
[9] Farmacopea de los EUA (USP 31). Formulario nacional. NF 26. Edición Anual Español. 2008. vol. 3. p. 3622-3623.
[10]HUNG JC, REDFERN MG, MAHONEY DW, et. al. Evaluation of Macroaggregated Albumin Particle Sizes for Use in Pulmonary Shunt Patient Studies. J Am. Pharm. Assoc. 2000; 40(1): 46-52.
[11]Manufacturing process to control particle size. United States Patent 6730286. 2004.
[12]LIN MS, KRUSE Sl, GOODWIN DA, et. al. Albumin loading effect: A pitfall in saline paper analysis of 99mTc albumin. Nucl. Med. 1974; 15(11): 1018-1020.
[13]DJOKI D, JANKOVI D, MAKSIN T. Radiochemical purity and particles number determinations of modified 99mTc-macroaggregated albumin. J. Serb. Chem. Soc. 2002; 67(8-9): 537-79.
[14]Farmacopea de los EUA (USP 30). Formulario nacional. NF 25. Edición Anual Español. 2007. vol. 1. p. 85-87, 105, 147.
[15]SAHA G. Characteristisc of specific radiopharmaceuticals. In: Fundamentals of Nuclear Pharmacy. Nueva York: Springer-Verlang; 1984. p. 105.
[16]Manual de Protocolos de Calidad de Radiofármacos. ARCAL XV. Producción y Control de Radiofármacos. 1999.
[17]MESA D.N. Evaluación de radiofármacos de alto y bajo peso molecular como radiolocalizador de procesos inflamatorios. La Habana: INEF, 2002. p.19-24.
[18]PACHECO C, VALLEJO JA, TORRES M, et. al. Alteración de la biodistribución de 99m Tc-microesferas de albúmina por su administración a través de una vena umbilical canalizada. Rev. Esp. Med. Nucl. 2000; 19(6): 434-436.
[19]ROBBINS PJ, FORTMAN DL, LEWIS JT. A new lung scanning agent. Appl. Radiat. Isot. 1973; 24(8): 481- 483.
[20]European Pharmacopeia. English 6 th edition. 2008. vol. 1. p. 1030-1031.
[21]SAHA G.B. Characteristics of specific radiopharmaceuticals. In : Fundamentals of Nuclear Pharmacy. Cap 7. Nueva York: Springer-Verlang, 1984. p. 105.
[22]HAMADA SE, MURAMATO E, BRITO HR, et. al. Producción de conjuntos de reactivos de macroagregados de albúmina de suero humano (MAA) marcados con 99m Tc destinado a gammagrafía pulmonar. IPEN-Pub-338. Sao Paulo. 1991.

Most read articles by the same author(s)